ub e2 Search Results


recombinant human/mouse/rat ubch5c/ube2d3 protein, cf  (Bio-Techne corporation)
94
Bio-Techne corporation recombinant human/mouse/rat ubch5c/ube2d3 protein, cf
Recombinant Human/Mouse/Rat Ubch5c/Ube2d3 Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human/mouse/rat ubch5c/ube2d3 protein, cf/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
recombinant human/mouse/rat ubch5c/ube2d3 protein, cf - by Bioz Stars, 2026-02
94/100 stars
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ub-conjugating (e2) enzyme  (Blackwell Science Ltd)
90
Blackwell Science Ltd ub-conjugating (e2) enzyme
Ub Conjugating (E2) Enzyme, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ub-conjugating (e2) enzyme/product/Blackwell Science Ltd
Average 90 stars, based on 1 article reviews
ub-conjugating (e2) enzyme - by Bioz Stars, 2026-02
90/100 stars
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e2–ub conjugates  (Siepmann GmbH)
90
Siepmann GmbH e2–ub conjugates
E2–Ub Conjugates, supplied by Siepmann GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e2–ub conjugates/product/Siepmann GmbH
Average 90 stars, based on 1 article reviews
e2–ub conjugates - by Bioz Stars, 2026-02
90/100 stars
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preloaded e2-ub  (Bristol Myers)
90
Bristol Myers preloaded e2-ub
Preloaded E2 Ub, supplied by Bristol Myers, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preloaded e2-ub/product/Bristol Myers
Average 90 stars, based on 1 article reviews
preloaded e2-ub - by Bioz Stars, 2026-02
90/100 stars
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e2~ub conjugate  (Deltex Medical)
90
Deltex Medical e2~ub conjugate
(A) Domain organisation of DELTEX family <t>E3s.</t> DTX1, DTX2 and DTX4 are classified into WWE domain-containing DELTEXes; DTX3 and DTX3L are classified into KH domain-containing DELTEXes. (B) DTX2-RD is not able to ubiquitylate nucleic acids. E21_DNA_A was incubated with were incubated with E1, E2, ATP, Ub and increasing amount of either DTX3L-RD or DTX2-RD, then the reactions were analysed on an SDS-PAGE gel and Urea gel and visualized using the Molecular Imager PharosFX system (BioRad). (C) Sequence alignment of the RING-DTC domains DELTEX E3s. Sequence conservation is colored in shades of red (red=identical, pink=conserved). The conserved catalytic residues in the DTC domain (DTX3L H707, E733) are indicated by asterisks. Differences in the RING domain and AR loop in DTC domain are indicated by green or black dashed box. Sequence alignment was generated using Clustal Omega and Jalview ( Waterhouse et al , 2009 ).
E2~Ub Conjugate, supplied by Deltex Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e2~ub conjugate/product/Deltex Medical
Average 90 stars, based on 1 article reviews
e2~ub conjugate - by Bioz Stars, 2026-02
90/100 stars
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rabbit reticulocyte e1 enzyme, e2 enzymes, isopeptidase t and ub-amc  (Boston Biochem)
90
Boston Biochem rabbit reticulocyte e1 enzyme, e2 enzymes, isopeptidase t and ub-amc
Fig. 6. BAP1 does not deubiquitylate BRCA1/BARD1 complex. (A) Recombinant BAP1 has C-terminal ubiquitin hydrolase activity. Recombinant BAP1 and the mutated protein BAP1(C91S) were incubated with <t>ubiquitin-AMC</t> as described in Materials and methods. Ubiquitin hydrolysis was measured by fluorescence using a Perkin Elmer Luminescence Spectrometer with excitation wavelength of 380 nm and emission wavelength of 460 nm. Fluorescence is plotted as arbitrary units (A.U.) (B) The 125I-polyubiquitylated form of BRCA1/BARD1 was isolated using nickel-NTA resin, washed in buffer and incubated with 100 nM BAP1 protein or 100 nM isopeptidase T as described in Materials and methods. Products were analysed by SDS–PAGE and deubiquitylation determined by the release of [125I]ubiquitin.
Rabbit Reticulocyte E1 Enzyme, E2 Enzymes, Isopeptidase T And Ub Amc, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit reticulocyte e1 enzyme, e2 enzymes, isopeptidase t and ub-amc/product/Boston Biochem
Average 90 stars, based on 1 article reviews
rabbit reticulocyte e1 enzyme, e2 enzymes, isopeptidase t and ub-amc - by Bioz Stars, 2026-02
90/100 stars
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e2 ub conjugating enzymes  (Genentech inc)
90
Genentech inc e2 ub conjugating enzymes
Fig. 6. BAP1 does not deubiquitylate BRCA1/BARD1 complex. (A) Recombinant BAP1 has C-terminal ubiquitin hydrolase activity. Recombinant BAP1 and the mutated protein BAP1(C91S) were incubated with <t>ubiquitin-AMC</t> as described in Materials and methods. Ubiquitin hydrolysis was measured by fluorescence using a Perkin Elmer Luminescence Spectrometer with excitation wavelength of 380 nm and emission wavelength of 460 nm. Fluorescence is plotted as arbitrary units (A.U.) (B) The 125I-polyubiquitylated form of BRCA1/BARD1 was isolated using nickel-NTA resin, washed in buffer and incubated with 100 nM BAP1 protein or 100 nM isopeptidase T as described in Materials and methods. Products were analysed by SDS–PAGE and deubiquitylation determined by the release of [125I]ubiquitin.
E2 Ub Conjugating Enzymes, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e2 ub conjugating enzymes/product/Genentech inc
Average 90 stars, based on 1 article reviews
e2 ub conjugating enzymes - by Bioz Stars, 2026-02
90/100 stars
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ub-conjugating enzyme e2 b  (Sangon Biotech)
90
Sangon Biotech ub-conjugating enzyme e2 b
Fig. 6. BAP1 does not deubiquitylate BRCA1/BARD1 complex. (A) Recombinant BAP1 has C-terminal ubiquitin hydrolase activity. Recombinant BAP1 and the mutated protein BAP1(C91S) were incubated with <t>ubiquitin-AMC</t> as described in Materials and methods. Ubiquitin hydrolysis was measured by fluorescence using a Perkin Elmer Luminescence Spectrometer with excitation wavelength of 380 nm and emission wavelength of 460 nm. Fluorescence is plotted as arbitrary units (A.U.) (B) The 125I-polyubiquitylated form of BRCA1/BARD1 was isolated using nickel-NTA resin, washed in buffer and incubated with 100 nM BAP1 protein or 100 nM isopeptidase T as described in Materials and methods. Products were analysed by SDS–PAGE and deubiquitylation determined by the release of [125I]ubiquitin.
Ub Conjugating Enzyme E2 B, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ub-conjugating enzyme e2 b/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
ub-conjugating enzyme e2 b - by Bioz Stars, 2026-02
90/100 stars
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thioester intermediate ub ~ e2  (High Energy Corporation)
90
High Energy Corporation thioester intermediate ub ~ e2
Fig. 6. BAP1 does not deubiquitylate BRCA1/BARD1 complex. (A) Recombinant BAP1 has C-terminal ubiquitin hydrolase activity. Recombinant BAP1 and the mutated protein BAP1(C91S) were incubated with <t>ubiquitin-AMC</t> as described in Materials and methods. Ubiquitin hydrolysis was measured by fluorescence using a Perkin Elmer Luminescence Spectrometer with excitation wavelength of 380 nm and emission wavelength of 460 nm. Fluorescence is plotted as arbitrary units (A.U.) (B) The 125I-polyubiquitylated form of BRCA1/BARD1 was isolated using nickel-NTA resin, washed in buffer and incubated with 100 nM BAP1 protein or 100 nM isopeptidase T as described in Materials and methods. Products were analysed by SDS–PAGE and deubiquitylation determined by the release of [125I]ubiquitin.
Thioester Intermediate Ub ~ E2, supplied by High Energy Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thioester intermediate ub ~ e2/product/High Energy Corporation
Average 90 stars, based on 1 article reviews
thioester intermediate ub ~ e2 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


(A) Domain organisation of DELTEX family E3s. DTX1, DTX2 and DTX4 are classified into WWE domain-containing DELTEXes; DTX3 and DTX3L are classified into KH domain-containing DELTEXes. (B) DTX2-RD is not able to ubiquitylate nucleic acids. E21_DNA_A was incubated with were incubated with E1, E2, ATP, Ub and increasing amount of either DTX3L-RD or DTX2-RD, then the reactions were analysed on an SDS-PAGE gel and Urea gel and visualized using the Molecular Imager PharosFX system (BioRad). (C) Sequence alignment of the RING-DTC domains DELTEX E3s. Sequence conservation is colored in shades of red (red=identical, pink=conserved). The conserved catalytic residues in the DTC domain (DTX3L H707, E733) are indicated by asterisks. Differences in the RING domain and AR loop in DTC domain are indicated by green or black dashed box. Sequence alignment was generated using Clustal Omega and Jalview ( Waterhouse et al , 2009 ).

Journal: bioRxiv

Article Title: Ubiquitylation of nucleic acids by DELTEX ubiquitin E3 ligase DTX3L

doi: 10.1101/2024.04.19.590267

Figure Lengend Snippet: (A) Domain organisation of DELTEX family E3s. DTX1, DTX2 and DTX4 are classified into WWE domain-containing DELTEXes; DTX3 and DTX3L are classified into KH domain-containing DELTEXes. (B) DTX2-RD is not able to ubiquitylate nucleic acids. E21_DNA_A was incubated with were incubated with E1, E2, ATP, Ub and increasing amount of either DTX3L-RD or DTX2-RD, then the reactions were analysed on an SDS-PAGE gel and Urea gel and visualized using the Molecular Imager PharosFX system (BioRad). (C) Sequence alignment of the RING-DTC domains DELTEX E3s. Sequence conservation is colored in shades of red (red=identical, pink=conserved). The conserved catalytic residues in the DTC domain (DTX3L H707, E733) are indicated by asterisks. Differences in the RING domain and AR loop in DTC domain are indicated by green or black dashed box. Sequence alignment was generated using Clustal Omega and Jalview ( Waterhouse et al , 2009 ).

Article Snippet: Mechanistically, DELTEX E3s recruit E2∼Ub conjugate and one NAD + or ADPr molecule using the RING and DTC domains, respectively.

Techniques: Incubation, SDS Page, Sequencing, Generated

Fig. 6. BAP1 does not deubiquitylate BRCA1/BARD1 complex. (A) Recombinant BAP1 has C-terminal ubiquitin hydrolase activity. Recombinant BAP1 and the mutated protein BAP1(C91S) were incubated with ubiquitin-AMC as described in Materials and methods. Ubiquitin hydrolysis was measured by fluorescence using a Perkin Elmer Luminescence Spectrometer with excitation wavelength of 380 nm and emission wavelength of 460 nm. Fluorescence is plotted as arbitrary units (A.U.) (B) The 125I-polyubiquitylated form of BRCA1/BARD1 was isolated using nickel-NTA resin, washed in buffer and incubated with 100 nM BAP1 protein or 100 nM isopeptidase T as described in Materials and methods. Products were analysed by SDS–PAGE and deubiquitylation determined by the release of [125I]ubiquitin.

Journal:

Article Title: Activation of the E3 ligase function of the BRCA1/BARD1 complex by polyubiquitin chains

doi: 10.1093/emboj/cdf691

Figure Lengend Snippet: Fig. 6. BAP1 does not deubiquitylate BRCA1/BARD1 complex. (A) Recombinant BAP1 has C-terminal ubiquitin hydrolase activity. Recombinant BAP1 and the mutated protein BAP1(C91S) were incubated with ubiquitin-AMC as described in Materials and methods. Ubiquitin hydrolysis was measured by fluorescence using a Perkin Elmer Luminescence Spectrometer with excitation wavelength of 380 nm and emission wavelength of 460 nm. Fluorescence is plotted as arbitrary units (A.U.) (B) The 125I-polyubiquitylated form of BRCA1/BARD1 was isolated using nickel-NTA resin, washed in buffer and incubated with 100 nM BAP1 protein or 100 nM isopeptidase T as described in Materials and methods. Products were analysed by SDS–PAGE and deubiquitylation determined by the release of [125I]ubiquitin.

Article Snippet: Rabbit reticulocyte E1 enzyme, E2 enzymes, isopeptidase T and Ub-AMC were purchased from Boston Biochem.

Techniques: Recombinant, Activity Assay, Incubation, Fluorescence, Isolation, SDS Page